RNA-seq: herramienta transcriptómica útil para el estudio de interacciones planta-patógeno
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El conocimiento del transcriptoma y su regulación es fundamental para la interpretación articulada de los diversos constituyentes moleculares que integran la red de respuesta génica ante un determinado evento inductor, como los que se presentan en interacciones planta-patógeno. La actual tecnología de secuenciación ha llevado al desarrollo del RNA-seq como herramienta transcriptómica que permite el secuenciamiento masivo de ADNc o ARN, y hace posible obtener perfiles de expresión génica de las respuestas de defensa, lo cual ofrece grandes posibilidades para profundizar en el entendimiento de los mecanismos que se activan durante las respuestas inmunes en plantas. El RNA-seq ha cambiado la manera de cómo se estudian los transcriptomas, y ha permitido identificar y cuantificar nuevos y conocidos transcriptos relacionados con defensa vegetal. Aquí se presenta el principio, aplicaciones y ventajas del RNA-seq; además se discuten trabajos recientes que revelan la importancia y utilidad de esta herramienta en estudios de interacciones planta patógeno.
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