Incidencia y caracterización molecular del Potato Leaf Roll Virus (PLRV) en las principales regiones productoras de papa de Colombia
Article Sidebar
Main Article Content
Abstract
Potato is one of the most important crops in Colombia, reaching about 162.000 ha and a production of more than 2.721.396 t. This crop is affected by various pests and diseases, including the tuber moths, late blight and different viruses. Virus diseases reduce yields and tuber quality of potato worldwide. PLRV is one of the most limiting viruses of potato in different latitudes, including the Andean region. This research evaluated the incidence and genotypic characteristics of PLRV in potato crops from the Colombian provinces of Antioquia, Boyacá, Cundinamarca and Nariño. Results indicated the occurrence of PLRV in the four provinces studied, with an average incidence of 20%. Molecular analysis of sequences generated from the viral capsid gene (CP) indicated a very low level of variation among isolates of this virus, and between them and the sequences of PLRV strains from different countries obtained from molecular databases. These results emphasize the need to strengthen phytosanitary measures in potato crops of Colombia.
Article Details
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
Those authors who have publications with this journal accept the following terms of the License Attribution-NonCommercial 4.0 International (CC BY-NC 4.0):
You are free to:
- Share — copy and redistribute the material in any medium or format
- Adapt — remix, transform, and build upon the material
The licensor cannot revoke these freedoms as long as you follow the license terms.
Under the following terms:
- Attribution — You must give appropriate credit, provide a link to the license, and indicate if changes were made. You may do so in any reasonable manner, but not in any way that suggests the licensor endorses you or your use.
- NonCommercial — You may not use the material for commercial purposes.
- No additional restrictions — You may not apply legal terms or technological measures that legally restrict others from doing anything the license permits.
The journal is not responsible for the opinions and concepts expressed in the works, they are the sole responsibility of the authors. The Editor, with the assistance of the Editorial Committee, reserves the right to suggest or request advisable or necessary modifications. They are accepted to publish original scientific papers, research results of interest that have not been published or sent to another journal for the same purpose.
The mention of trademarks of equipment, instruments or specific materials is for identification purposes, and there is no promotional commitment in relation to them, neither by the authors nor by the publisher.
How to Cite
References
Büchen-Osmond, C.: «The Universal Virus Database of the International Committee on Taxonomy of Viruses», www.ictvdb.org (consultado en julio de 2010).
Felsenstein, J.: «Confidence Limits on Phylogenies: An Approach Using the Bootstrap», Evolution 39:783-791, EE. UU., 1985.
Guerrero, O.; G. Martínez: «Evaluación de pérdidas ocasionadas en la variedad de papa Puracé por los virus Potato virus X, Potato virus Y y Potato leafroll virus», Fitopatol. Colomb. 9:3-40, Colombia, 1980.
Guyader, S.; D. G. Ducray: «Sequence Analysis of Potato leafroll virus Isolates Reveals Genetic Stability, Major Evolutionary Events and Differential Selection Pressure Between Overlapping Reading Frame Products», J. of Gen. Virology 83:1799-1807, Inglaterra, 2002.
Guzmán, M.; M. Caro; Y. García: «Validación de la técnica serológica de inmunoimpresión para detección de diferentes virus que afectan el cultivo de papa», I Taller Nacional sobre Patógenos del Suelo, Virus e Insectos Plaga Diferentes a Tecia solanivora, Bogotá, Colombia, 2004, p. 41.
Guzmán, M.; V. Román; L. Franco; P. Rodríguez: «Presencia de cuatro virus en algunas accesiones de la Colección Central Colombiana de papa mantenida en campo», Agron. Col. 28:225-233, Colombia, 2010.
Matthews, R. E. F: Diagnosis of Plant Virus Diseases. CRC Press, Canadá, 1993.
Ministerio de Agricultura y Desarrollo Rural: «Observatorio Agrocadenas. Cuarto informe de coyuntura 2006», http://www.agrocadenas.gov.co/documentos/coyuntura/Inf_Coyuntura_papa_4.pdf (consultado en: marzo de 2009).
Plchova, H.; N. Cerovska; T. Moravec; P. Dedic: «Molecular Analysis of Potato leafroll virus Isolates from the Czech Republic», Virus Genes 39:153-155, Springer-Verlay; Nueva York; Heidelberg, Berlín, 2009.
Pooramini, N.; J. Heydarnejad; H. Massumi: «Incidence and Molecular Analysis of Potato leafroll virus in Iran», J. Phytopathol. 158:182- 185, Alemania, 2010.
Rahman, M. S.; A. M. Akanda: «Effect of PLRV Infected Seed Tuber on Disease Incidence, Plant Growth and Yield Parameters of Potato», Bangladesh J. Agril. Res. 35:359-366, Bangladesh, 2010.
Salazar, L. F.: Los virus de la papa y su control, CIP, Lima, 1995.
Salazar, L. F.: «Emerging and Re-Emerging Potato Diseases in the Andes», Potato Res. 49:43-47, EE. UU., 2006.
Sánchez de Luque, C.; P. Corzo; O. Pérez: «Incidencia de virus en papa y su efecto sobre rendimientos en tres zonas agroecológicas de Colombia», Rev. Lat. Papa 4:36-51, Perú, 1991.
Singh, R. P.; J. Kurz; G. Boiteau; G. Bernard: «Detection of Potato leafroll virus in Single Aphids by the Reverse Transcription Polymerase Chain Reaction and Its Potential Epidemiological Application», J. of Virol. Methods 55:133-143, EE. UU., 1995.
Swofford, D. L.: PAUP: Phylogenetic Analysis Using Parsimony (*and Other Methods), Version: 4, Sinauer Associates, Sunderland, EE. UU., 1998.
Taliansky, M.; M. A. Mayo; H. Barker: «Potato leafroll virus: a Classic Pathogen Shows New Tricks», Mol. Plant Pathol. 4:81-89, Inglaterra, 2003.
Zapata, J. L.: «Algunos aspectos sobre los virus de la papa en Colombia», I Taller Nacional sobre Patógenos del Suelo, Virus e Insectos Plaga Diferentes a Tecia solanivora, Colombia, 2004, pp. 24-40.