Evaluación del método de conservación en papel de filtro en dos cepas de Bacillus subtilis Cohn, mediante la actividad antagónica frente a Rhizoctonia solani Kühn
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Abstract
Antagonistic activity of two Bacillus subtilis strains preserved in filter paper disks at 4°C for a period of three years was evaluated. Strains Bs-21 and Bs-42, isolated from Cuban soil and antagonists to Rhizoctonia solani Rs-10 were choiced. Five colonies from Bs-42 and one from Bs-21, randomly selected were growth in nutrient agar. The antagonism was compared by dual culture method in PDA after 96 h at 30°C. Viability of both strains was 108 CFU/mL. Selected colonies kept purity, morphological characteristics and positive response to Gram stain compared with B. subtilis ATCC 6633. All selected colonies inhibited mycelial growth of R. solani Rs-10, but not the reference strain. Strain Bs-21 had the lower inhibition (78%) while Bs-421 and Bs-422 had the higher percent (98%). Variance analysis showed significant differences in the percentage of inhibition exhibited by tested colonies.
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References
Basurto, M. G.; M. I. Font; J. García; M. Vásquez: «Cambios en la estructura celular durante la actividad antagónica de Bacillus subtilis contra Rhizoctonia solani y Fusarium verticillioides», Acta Microscópica 19(2):138-144, España, 2010.
Carreras, B.: «Aislamiento y determinación de las características morfológicas, moleculares y patogénicas de cepas nativas de la Bacteria Bacillus thuringiensis Berliner con potencial para el control de plagas», tesis en opción al grado científico de Doctora en Ciencias Biológicas, Inisav, La Habana, 2007.
Castro, G.; J. T. Hernández; C. Aquino: «Manual sobre conservación de microorganismos», Instituto Politécnico Nacional, Escuela Nacional de Ciencias Biológicas, México, 2000.
Claus, D.; R. C. Berkeley: «Genus Bacillus Cohn 1872», Bergey’s Manual of Systematic Bacteriology, Williams y Wilkins Edit., Baltimore, EE. UU., 1986, pp. 1105-1139.
García, M. D.; F. Uruburu: «La conservación de cepas microbianas», Colección Microbiana de Cultivos Tipo (CECT) Universidad de Valencia, España, Temas de la actualidad, 2001, http://www.cect.org/docs/instrseguridad.pdf (consultado el 20 de diciembre de 2008).
Marín, D.; A. Fernández: «Elementos para el manejo de patógenos fúngicos en semilleros de tabaco en la tecnología de bandejas flotantes», tesis de Maestría en Microbiología, Universidad de La Habana, Inisav, La Habana, 2003.
Ramírez, Y.; V. Pazos; I. Sánchez: «Selección de rizobacterias antagonistas con potencial para el control biológico de las enfermedades producidas por Acidovorax avenae subsp. Citrulli, Xanthomonas cucurbitae y Fusarium oxysporum», tesis de Maestría en Microbiología General, Centro de Ingeniería Genética y Biotecnología de Camagüey, Cuba, 2005.
Sosa, A., V. Pazos; M. González: «Nuevos aislados de Bacillus spp. antagonistas a Sclerotium rolfsii, Rhizoctonia solani y Pythium aphanidermatum», Fitosanidad 10(1):73-74, La Habana, 2006.
Uzunova-Doneva, T.; T. Donev: «Anabiosis and Conservation of Microorganisms», Journal of Culture Collections 4:17-28, Bulgaria, 2004-2005, http://hdl.handle.net/1807/5199 (consultado en enero de 2011).