Aislamiento de nematodos entomopatógenos en áreas de Buenaventura, Valle del Cauca, Colombia
Main Article Content
Abstract
A study to identify entomopathogenic nematodes in three localities of the special district of Buenaventura, Valle del Cauca, Colombia (Cisneros, Córdova, Llano Bajo) was conducted, and the technique of insect-tramp with larvae of Galleria mellonella (L.) was used The Isolated entomopathogenic nematodes were inoculated again in larvae of G. mellonella in order to assess its pathogenicity and to obtain samples for his identification to genus level. Entomopathogenic nematodes were detected in 36.84 % of samples and only genus Steinernema was positive in identification. Isolated nematodes of three localities caused 100 % mortality in the larvae of G. mellonella at 48 hours. Results indicate that the genus Steinernema is present in the three locations of Buenaventura. This result opens possibilities for evaluation and subsequent use in integrated management programs of insect pests with economic importance in the region.
Article Details

This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
Those authors who have publications with this journal accept the following terms of the License Attribution-NonCommercial 4.0 International (CC BY-NC 4.0):
You are free to:
- Share — copy and redistribute the material in any medium or format
- Adapt — remix, transform, and build upon the material
The licensor cannot revoke these freedoms as long as you follow the license terms.
Under the following terms:
- Attribution — You must give appropriate credit, provide a link to the license, and indicate if changes were made. You may do so in any reasonable manner, but not in any way that suggests the licensor endorses you or your use.
- NonCommercial — You may not use the material for commercial purposes.
- No additional restrictions — You may not apply legal terms or technological measures that legally restrict others from doing anything the license permits.
The journal is not responsible for the opinions and concepts expressed in the works, they are the sole responsibility of the authors. The Editor, with the assistance of the Editorial Committee, reserves the right to suggest or request advisable or necessary modifications. They are accepted to publish original scientific papers, research results of interest that have not been published or sent to another journal for the same purpose.
The mention of trademarks of equipment, instruments or specific materials is for identification purposes, and there is no promotional commitment in relation to them, neither by the authors nor by the publisher.
How to Cite
References
Ansari, M. A.; L. Waeyenbergen; M. Moens: «First Record of Steinernema glaseri Steiner, 1929. (Rhabditida: Steinernematidae) from Belgium: a Natural Pathogen of Hoplia philanthus (Coleoptera: Scarabaidae)», Nematology 7 (6): 953-956, Inglaterra, 2005.
Boemare, N.: Biologgy, Taxonomy and Systematic of Photorabdus and Xenorthabdus, Entomopathogenic Nematology, New Jersey, Rutgers University, EE. UU., 2002, pp. 35 y 36.
Byron, J. A.; B. N. Khuong: Entomopathogenic Nematology. Taxonomy and Systematics, CAB International, Entomology and Nematology Department, University of Florida, EE. UU., 2002.
Caicedo, A. M.; H. Trujillo; M. P. Quintero; P. Calatayud; A. Bellotti: «Reconocimiento de nematodos entomopatógenos asociados a Cyrtonemus bergi en tres localidades de Colombia», Memorias del XXXI Congreso de La Sociedad Colombiana de Entomología, julio 26- 30, Bogotá, Colombia, 2004.
Constantino, L. M.; S. H. Caicedo; C. A. Torres: «Manejo integrado del barrenador del fruto del chontaduro Palmelampius heirichi con pequeños productores del municipio de Guapi-Cauca», Fundación Levante en marcha, PRONATTA, Informe Técnico, Cali, 2003.
García, O. T.; Fernández, E. G.; A. Schesteperov: «Indicaciones metodológicas para la detección y evaluación de las infestaciones de nematodos parásitos en cultivos agrícolas», Información Técnica, año II, no. 4, Inisav, La Habana, 1979.
González, R. T.; D. Torres; V. H. Moreno; F. Molineros: «Viveros del Pacífico», Agrupación de Viveristas del Consejo Comunitario del Alto y medio río Dagua. Universidad del Pacífico- Fundación Danza & Vida-Corporación Autónoma Regional del Valle del Cauca, 2009.
Griffin, C. T.; S. A. Joyce; I. Dix; A. M. Burnell; M. J. Downes: «Characterization of Heterorhabditis (Nematoda: Heterorhabditidae) for Ireland and Britain by Molecular and Cross-Breeding Techniques and the Occurrence of the Genus in These Islands», Fundamental and Applied Nematology 17: 245-243, Inglaterra, 1994.
Gyayetto, A. L.; L. I. Chichon: «Distribución, gama de huéspedes y especificidad de cinco poblaciones de Heterorhabditis (Nematoda: Heterorhabditidae) del Alto Valle del Río Negro y Neuquén, Argentina», RIA. 35 (2): 163-183, INTA, Argentina, 2006.
Hara, A. H.; R. Gugler; H. K. Kaya; L. M. Lebeck: «Natural Populagtikons of Entomopathogenic Nematodes (Rhabditida: Steinernematidae) from the Hawaiian Islands», Environmental. Entomology 17: 211-216, EE. UU., 2002.
Haukeland, S.; A. Otto; N. Hauge: «The Effect of Temperature on the Efficacy of Steinernematid and Heterorhabditid Nematodes», XXV Annual Meeting Society for Invertebrate Pathology, Heidelberg, 1992, p. 98.
Hazir, S.; K. Nevin; S. P. Stock; H. K. Kaya; O. Servent: «Diversity and Distribution of Entomopathogenic Nematodes (Rhabditida: Steinernemidae and Heterorhabditidae) in Turkey», Biodiversity and Conservation 12: 375-386, Alemania, 2003.
Hernández, P.; J. Alcázar; S. Garcés; P. Gallegos: «Prospección de nematodos entomopatógenos para el control del gusano blanco Prenotrypes vorax (Hustache) (Coleoptera:Curculionidae) en Ecuador», http://www.redpav-fpolar.info.ve/ventomol/v13-2/132b0002, 2005.
Hominick, W. M.; M. Bricoe: «Ocurrence of Entomopathogenic Nematodes (Rhabditida: Steinernematidae y Heterorhabditidae) in Bristish soils»•, Parasitology 100: 295-302, Inglaterra, 1990.
Hominick, W. M.: «Biogeography», Entomopathogenic Nematotoly, CAB International, New Jersey, Rutgers University, EE. UU., 2002, pp. 115-143.
Kaya, H. K.: «Entomogenous Nematodes for Insect Control in IPM Systems», Biological Control in Agricultural IPM Systems, Academic Press, Nueva York, 1985, pp. 283-302.
Kaya, H. K.: «Soil Ecology», Entomopathogenic Nematodes in Biological Control, CRC Press, Boston, EE. UU., 1990, pp. 923-1011.
Koppenhofer, A. M.; H. K. Kaya; S. P. Taormino: «Infectivity of Entommopathogenic Nematodes (Rhabditida: Steinernematidae) at Different Soil Depths and Moistures», Journal Invertebrate Pathology 65: 193-199, Holanda, 2006.
Kung, S. P.; R. Gaugler; H. K. Kaya: «Influence of Soil pH and Oxygen on Persistence of Steinernema spp.», Journal of Nematology, Lakelaand 22 (4): 440-445, EE. UU., 1990.
Long, K. P.; S. Takemoto; K. Futai: «Steinernema ashiuenses sp. n (Nematoda:Steinernemidae) a New Entomopathogenic Nematode from Japan», Nematology 8 (5): 681-690, Inglaterra, 2006.
Molina, A.; N. J. López: «Producción in vitro de tres entomonematodos con dos sistemas de infección en dos hospedantes», Revista Colombiana de Entomología 27 (1-2): 723-778. 2002.
Peña, R. E.; C. R. Reyes; P. S. Bastidas: «Reconocimiento del daño y manejo del insecto Palmelampius heirichi, barrenador del fruto del chontaduro en la costa pacífica colombiana», Corpoica, Boletín de Divulgación no. 16, Tumaco-Nariño, 2002.
Poinar, G. O.: «Taxonomy and Biiology of Steinernematidae and Heterhorabditidae», Entomopathologenic Nematodes in Biological Control, CRC Press, Boca Ratón, Florida, EE. UU., 1990, pp. 75-87.
Portillo, A. C.; G. M. Villani; J. M. Tauber; A. C. Tauber; P. J. Nyrop: «Entomopathogenic Nematode (Rhabditida: Heterorhabditida Steinernematidae) Response to Soil Texture and Bulk Density», Environmental Entommology, Lanham 28 (2): 123-126, EE. UU., 2000.
Qiu, L. Hu; X. Zhou; Y. Pang; K. B. Nguyen: «Steinernema beddingi n. sp. (Yunnan, China)», Nematology 7 (5): 737-748, Inglaterra, 2005.
Rolston, A.; C. T. Griffin; D. Downes: «Distribution of Entomopathogenic Nematodes in Indonesia. Journal Helminthol 7 (2): 259-266, EE. UU., 2005.
Rosa, J. S.; E. Bonifassi; J. Amaral; L. A. Lacey; N. Simoes; C. Laumond: «Natural occurrence of Entomopathogenic (Rhabditidae: Steinr- nematidae) in the Azores», Jour. Nematol. 32: 215-222, EE. UU., 2000.
Rosales, L.; Z. Suárez: «Nematodos entomopatógenos como posibles agentes de control del gorgojo negro del plátano Cosmopolites sordidus (Germar, 1824) (Coleoptara:Curculionidae)», Bol. Entomol. Venez. 13 (2): 123-140, 1998.
Simard, L.; G. Belair; S. P. Stock; H. Mauleon; J. Dionne: «Natural Occurrence of Entomopathogenic Nematodes (Nematidae: Steinernematidae) on Golf Courses in Eastern Canada», Nematology 9 (3): 325-332, Inglaterra, 2007.
Stock, P.: «Sistemática y biología de nematodos parásitos asociados a insectos de importancia económica», Universidad Nacional del Litoral, Esperanza, Santa Fe, Argentina, 1998.
Stock, S. P.; D. Hunt: «Morphology and Systematics of Nematodes Used in Biocontrol. Nematodes as Biocontrol Agents, CABI Publishing, Inglaterra, pp. 3-43, 2005.
