Entomopathogenic fungi Beauveria bassiana (Bals.-Criv.) Vuill for the biological control of trips (Thysanoptera: Thripidae) in potato crop (Solanum tuberosum L.) in Cuba

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Ana Ibis Elizondo Silva
Yamilé Baró Robaina
Yohana Gato Cárdenas
Yuramis Quesada Mola
Enrique Quesada Mola
Enrique Ponce Grijuela
Miguel Milán Labrada
Manuel teje

Abstract

In Cuba biopesticides application is one of the diverse tactics of management that are used for the control of Thrips palmi Karny in potato crop (Solanum tuberosum L.). These biopesticides is been of a rigorous selection of strains by means of research that demonstrate the possibility of it use in potato crop. For it and with the objective to evaluate the effect of two strains of the entomopathogenic fungi Beauveria bassiana (Bals.-Criv.) Vuill it has more than enough trips, laboratory experiments and fields were developed. For the experiment in vitro nymphs were selected of first and second instar that later on were inoculated with the strains LBBb - 1234 y LBBb-1, this last one as comparison variant. The inoculation was carried out with 1 suspension ml for each strain to the concentration of 109 conidios • mL-1, as well as a witness tried with sterile water. The evaluations were carried out at 24, 48, 72, 96 and 120 hours, quantifying the alive and dead individuals. The experiment in vivo was carried out at random in field parcels with a block design with three variants, LBBb-1234, LBMa-11 one as comparison variant and a witness without treatment. The laboratory results showed that B. bassiana presented mortality to 24 hours after the treatment. The biggest percentage of mortality reached it the strain LBBb-1234 with a value of 70 % at the 120 hours after the treatment. The field results showed that the population of nymphs decreased after carrying out the second treatment with the biopesticides.

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Elizondo Silva, A. I., Baró Robaina, Y., Gato Cárdenas, Y., Quesada Mola, Y., Quesada Mola, E., Ponce Grijuela, E., Milán Labrada, M., & teje, M. (2018). Entomopathogenic fungi Beauveria bassiana (Bals.-Criv.) Vuill for the biological control of trips (Thysanoptera: Thripidae) in potato crop (Solanum tuberosum L.) in Cuba. Fitosanidad, 22(1), 5-10. https://www.fitosanidad.edicionescervantes.com/index.php/fitosanidad/article/view/34
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Original Articles

How to Cite

Elizondo Silva, A. I., Baró Robaina, Y., Gato Cárdenas, Y., Quesada Mola, Y., Quesada Mola, E., Ponce Grijuela, E., Milán Labrada, M., & teje, M. (2018). Entomopathogenic fungi Beauveria bassiana (Bals.-Criv.) Vuill for the biological control of trips (Thysanoptera: Thripidae) in potato crop (Solanum tuberosum L.) in Cuba. Fitosanidad, 22(1), 5-10. https://www.fitosanidad.edicionescervantes.com/index.php/fitosanidad/article/view/34

References

Bhattacharryya, B.; A. Baruah; D.Purnima; U. Bhuyan. 2008. Field efficacy of Beauveria bassiana (Bals.-Criv.) Vuill and Metarhizium anisopliae (Metschn.) Sorki (Deuteromycotina: Moniliales) Against White Grubs in Assam. Journal of Biological Control 22 (1): 81-64. India.

Bustillo, A E. 2009. Evaluación de insecticidas químicos y biológicos para controlar Frankliniella occidentalis (Thysanoptera: Thripidae) en cultivos de espárragos. Revista Colombiana de Entomología 35 (1): 12-17. Colombia.

Ciba-Geigy. 1981. Manual para ensayos de campo en protección vegetal, Werner Püntener, División Agricultura, 2 ed. CIBA-GEIGY, 205 p. Suiza. Cuba.

Devi, U.; K. Padmavathi; J.U. Maheswara; C. Akbar; M. Mohan. 2008. A study of Host Specifity in the Entomopathogenic Fungus Beauveria bassiana (Hypocreales: Clvicipitaceae). Biocontrol Science and Thecnology 18: 975-989. Inglaterra.

Elizondo, A I, Murguido, C A y Matamoros, M. 2011. Patogenicidad de los hongos Metarhizium anisopliae (Metschn.), Lecanicillium lecanii (Zimm.) Zare & Gams y Beauveria bassiana (Bals.-Criv.) Vuill. sobre Thrips palmi Karny en el cultivo de la papa (Solanum tuberosum L.). Fitosanidad 15 (3): 147-151. La Habana, Cuba.

Elizondo, A I, Murguido, C A, Milán, M, Montero, J y Mirabal, L. 2013. El insecticida imidacloprid y los bioplaguicidas para el control de Thrips palmi Karny en el cultivo de la papa (Solanum tuberosum L.). Fitosanidad 17 (1): 31-34. La Habana, Cuba.

Elizondo, A I, Y. Baró, Y. Gato, Y. Quesada, E. Ponce, M. Milán y M. Tejeda. 2017. El hongo entomopatógeno Metarhizium anisopliae (Metschn.) una alternativa para el control biológico de trips (Thysanoptera: Thripidae) en el cultivo de la papa (Solanum tuberosum L.) en Cuba. Fitosanidad 21 (3): 111-114. La Habana, Cuba.

Elósegui, O y Elizondo, A I. 2010. «Evaluación microbiológica in vitro de mezclas de especies de hongos entomopatógenos, ingredientes activos de bioplaguicidas cubanos», Fitosanidad 14 (2): 103-109. La Habana, Cuba.

Fargues, J., Maniania N.K., Delmas J.C., Smits, N. 1992. Influence de la temperature sur la croissance in vitro d’Hyphomycetes entomopathogenes. Agronomie. 12:557-564.

Helyer, N. L. y Brobyn, P J. 2008. Chemical control of western flower thrips (Frankliniella occidentalis Pergande). Annals of Applied Biology 121 (2): 219-231. México.

Lecuona, R. 1996. Microorganismos patógenos empleados en el control microbiano de insectos plaga. Buenos Aires, Argentina. 338p.

Lopes Da Silva, A.; Da Silva, N.; F.; Pires, L.; Ferreira, H.; Caetano, V.; Peixoto, L. 2003. Eficiência agronômica de inseticidas no controle do Thrips tabaci Lind., (Thysanoptera, Thripidae) na cultura do alho. Pesquisa Agropecuária Tropical 33 (1): 39-42. Brasil.

Morales, A., D., D.; Jarquín, G. R.; Gómez, J., R.; Díaz, O., G. y Marín, S. 2014. Evaluación de un formulado vegetal de Beauveria bassiana en condiciones de laboratorio para el control de la broca del café. Fitosanidad. 18, (1): 5-14. La Habana, Cuba.

Shirotsuka, K, Hamasaki, K, Shibao, M and Okada, K. 2016. ¨Control of melon thrips, Thrips palmi Karny, on greenhouse cucumber with the combined use of a red insect-proof net, Amblyseius swirskii, and Metarhizium anisopliae¨. Ann. Rept. Kansai Pl. Prot. (58): 45-49. Japón.

Suárez, G.H., D. 2009. Patogenicidad de Beauveria bassiana (Deuteromycotina: Hyphomycetes) sobre Sitophilus zeamais (Motschulsky) (Coleoptera: Curculionidae) plaga de maíz almacenado. Revista Intropica 45-51. Colombia.

Walstad, J.; Anderson R.; Stambaugh W. 1969. Effects of environmental conditions on two species of muscardine fungi (Beauveria bassiana and Metarrhizium anisopliae). Journal of Invertebrate Pathology. 16(2): 221-226.