Influencia de la carga microbiana contaminante inicial del sustrato en la calidad final de biopreparados de Trichoderma harzianum rifai y Beauveria bassiana (balsamo) vuillemin
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Abstract
The sterility achieved at differing sterilization regimes in substrates used for both Beauveria and Trichoderma mass production was studied as well as its influence on product quality in mass production centres of western Cuba. It was demonstrated that the sterilization regimes should be adjusted according to the contamination level of the substrate. A contamination level of 107 UFC/g or greater was found in 92% of Trichoderma samples. In contrast, 23% of Beauveria samples had a contamination level of 107 UFC/g meanwhile the rest of the samples had a lower contamination level. For Trichoderma products a decrease in viability was observed for the samples having contamination levels of 108 UFC/g, which belonged to the production runs developed on contaminated substrate.
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References
Fernádez-Larrea, Orietta: Temas interesantes acerca del control microbiológico en Cuba, INISAV, La Habana, 2001.
Hall, R.; Colectivo Investigadores Laboratorio Provincial Sanidad Vegetal Villa Clara y Subdirección Protección Vegetal Centro Nacional Sanidad Vegetal: «Metodología para la reproducción de hongos entomopatógenos con calidad alta y estable de forma artesanal», Centro Nacional de Sanidad Vegetal, La Habana, 2000.
Hong, T. D.; J. Jun; R. H. Ellis; N. E. Jenkins; D. Moore: «The Effect of Storage Environment on the Longevity of Conidia of Beauveria bassiana», Mycological Research 105:597-602, 2001.
Jenkins, N. E.; D. Grzywacz: Quality Control of Fungal and Viral Biocontrol Agents-Assurance of Product Performance», Biocontrol Science and Technology 10:753-777, 2000.
Jenkins, N. E.; D. Grzywacz: «Towards The Standardization of Quality Control of Fungal and Viral Biocontrol Agents». Chapter 18, Quality Control and Production of Biological Control Agents: Theory and Testing Procedures, CAB International, 2003, pp. 247-262.
Jenkins, N. E.; G. Heviefo; J. Langewald; A. J. Cherry; C. J. Lomer: «Development of Mass Production Technology for Aerial Conidia for Use As Mycopesticides», Biocontrol News and Information 19, 21N-31N, 1998.
Lecuona, R.: Microorganismos patógenos empleados en el control de insectos plaga, Talleres Gráficos Mariano Mas, Buenos Aires, 1996.
Manual BIOCEN de medios de cultivo, 2a. ed., Centro Nacional de Biopreparados, La Habana, 2001.
Moore, D.; P. M. Higgins: «Viability of Stored Conidia of Metarhizium flavoviride Gams and Rozsypal, Produced Under Differing Culture Regimes and Stored with Clays», Biocontrol Science and Technology 7:335-343, 1997.
Norma Cubana para la Producción de Bioplaguicidas, MINAGRI, Cuba, 1993.
NC 72-02. Norma Cubana Biopreparados de Entomopatógenos. Métodos de ensayo. Biotecnología Agrícola, Cuba, 1993.
NC 72-03. Norma Cubana Biopreparado del Entomopatógeno Verticillium lecanii. Especificaciones. Biotecnología Agrícola, 1993.
NC 72-04. Norma Cubana Biopreparado del Entomopatógeno Metarhizium anisopliae. Especificaciones. Biotecnología Agrícola, Cuba, 1993.
NC 72-05 Norma Cubana Biopreparado del Entomopatógeno Beauveria bassiana. Especificaciones. Biotecnología Agrícola, 1993.
