Ensayo en fragmentos de hojas de bananos y plátanos (Musa spp.) para el estudio a nivel monocíclico de la evolución de los síntomas de la sigatoka negra causada por Mycosphaerella fijiensis morelet
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Abstract
An assay of artificial inoculation on banana leaf fragments in vitro was studied. Fragments of 3.5 x 3.5 cm of the first fully expanded leaf (10 fragments/cultivar) were washed with sterilized water and placed in 10 cm Petri dishes containing 20 g agar. L–1 + 40 µg.mL–1 of benzimidazol. The fragments were inoculated with different concentrations of M. fijiensis conidia obtained from in vitro cultures the pathogen and were incubated under fluorescent light. The number of lesions and its evolution to different stages of development were assessed daily in a single infection cycle. The optimal conidial concentration for the development of the assay was determined. A strong correlation between the curves of development of lesions on time in the fragments in vitro at monocyclic level and the curves of development of the disease on time in different cultivars at polycyclic level in the field was observed allowing sustain that the method is useful to compare the level of partial resistance of Musa cultivars to Black Sigatoka disease and the aggressiveness of the populations under controlled conditions. The duration of the fragments green life in vitro in the experiments do not allowed assess the intensiveness of the sexual reproduction of the pathogen. Further research is required to increasing the green life of the fragments of banana leaves to allowing the formation of the sexual reproduction of the pathogen in the spots.
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References
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